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Submission: On July 29 via manual from US — Scanned from DE
Submission: On July 29 via manual from US — Scanned from DE
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</form>Text Content
You need to enable JavaScript to run this app. Press Release Next-Generation Manufacturing Facility Case Study Enabling Rapid Target Validation for Therapeutics Featured CRISPR Conversation with QBI Leaders Company * Platforms Overview Halo Eclipse * Company About Quality Newsroom Careers Partnerships Global Distributors Contact Support * Help Center Ordering Product Info Analyzing Results Troubleshooting Tips and Tricks Help Center * Get in Touch Contact Order * Edited Cells Knockout Pool Knockout Clone * Screening Custom Library * DIY CRISPR Synthetic sgRNA Knockout Kit Cas9 Nuclease -------------------------------------------------------------------------------- Subscribe Cart Your Orders Account Sign In Products Workflows Learn Schedule a Consultation Contact Sales -------------------------------------------------------------------------------- AN INSIDE LOOK AT WHAT’S HAPPENING AT THE BENCHTOP CRISPR Benchmark Report - Vol. 01 CRISPR IS EASILY ONE OF THE MOST POPULAR TOOLS IN MODERN BIOLOGY. SINCE ITS INTRODUCTION IN 2012, CRISPR-BASED PUBLICATIONS HAVE INCREASED ABOUT 30 FOLD. WITH NIH FUNDING FOR CRISPR-RELATED PROJECTS PER YEAR CROSSING A BILLION DOLLARS IN 2020, IT IS SAFE TO ASSUME THAT MORE AND MORE RESEARCHERS WILL ADOPT CRISPR IN THEIR PROJECTS IN THE COMING YEARS. KNOCK KNOCK, IT’S CRISPR Given that CRISPR has been around less than a decade, researchers have had to learn it on-the-go while trying to maintain the pace of their research. This is no small feat. The CRISPR workflow is a complex machine; every tiny component has to work perfectly for the machine to run smoothly. A small mistake in any of the multiple steps in a CRISPR experiment could cost months of wasted time and resources. We wanted to understand the expertise, experience, and attitude of CRISPR researchers towards the experiments. THE METHODOLOGY To find answers to these pressing questions, we conducted a blind online survey through various third-party life science publications that cater to a scientific audience. The survey contained 36 questions covering researchers’ challenges, applications, success levels, and satisfaction levels around the CRISPR workflow. The CRISPR Benchmark Report highlights the informative trends that emerged from the survey. DOWNLOAD NOW -------------------------------------------------------------------------------- Title* Please SelectExecutive ManagementDirector Head of DepartmentPrincipal InvestigatorGroup LeaderPrincipal ScientistLab ManagerScientistResearch AssociatePostdocGraduate StudentProcurementStudent (Here to Learn)Not a Scientist First Name* Last Name* Email* Application Area* Please SelectDisease ModelingPathway AnalysisCell and Gene TherapyDiagnosticsScreening and Target IDTarget ValidationBioengineeringEpigeneticsGene Function Other Are you using CRISPR?* Please SelectCurrently using CRISPRPlan to use CRISPR within 6 monthsPlan to use CRISPR in 6+ monthsNo plans to use CRISPR Requested Action* Please SelectI’m Ready to Buy NowSend Me a QuoteTalk to a Technical ExpertRequest a Seminar for My LabNone at this TimeJust Here to Learn THE CRISPR WORKFLOW IS A COMPLEX MACHINE; EVERY TINY COMPONENT HAS TO WORK PERFECTLY FOR THE MACHINE TO RUN SMOOTHLY. THE GOAL A successful experiment requires several considerations around guide design, cell type optimization, and transfection method. Yet, researchers have to balance complicated CRISPR workflows in the lab while managing their project goals and deadlines. We wanted to dig into this paradoxical situation by getting an inside look at what’s happening with CRISPR in today’s lab. The following were our main objectives for conducting the survey. KNOW THE RESEARCHERS Information around respondents’ professional backgrounds, areas of interest, years of expertise, and type of CRISPR editing helped us ensure that we collected unbiased data spread out over diverse educational and research labs. UNDERSTAND THE CHALLENGES That CRISPR is tedious is common information, but we wanted to dig into specifics: how much time do researchers invest in each step of the experiment, what parts do they find challenging, and what results do they get in the end. UNCOVER THE PERCEPTIONS Given that researchers are inclined to invest in a CRISPR experiment, despite the heavy lifting, we were curious if they found the ordeal worth it. The last part of the survey queried the satisfaction levels of researchers around their CRISPR results. ONLY 15% OF CRISPR RESEARCHERS ARE VERY SATISFIED WITH THEIR RESULTS. THE FINDINGS CRISPR RESEARCH IS DIVERSE We found that CRISPR researchers worked in diverse applications & research areas. Interestingly, irrespective of the type of research, knockouts emerged as the most popular editing method. DO-IT-YOURSELF CRISPR IS HARD It became clear from the data that DIY CRISPR is hard. Researchers need 7 attempts, on average, before they succeed, and especially struggle with the optimization step. Even when they do get an edit, they report a sub-optimal average editing efficiency of 43%. RESEARCHERS ARE NOT SATISFIED WITH RESULTS With average editing efficiency ranging at 43%, it came as no surprise that only 15% of CRISPR researchers are very satisfied with their results. Researchers also fall short of their ideal goals; they report the desire to study 17 gene targets in parallel, but end up studying just 7 targets. RESEARCHERS SPEND ABOUT 472 HOURS OF HANDS-ON TIME AND $18,394 OF TOTAL COSTS ON PERFORMING A CRISPR EXPERIMENT IN THEIR LAB. THE TRUE COSTS OF CRISPR Our calculations, based on the survey data inputs, showed that the overall time and monetary investment in CRISPR is quite high. Researchers may not realize the financial burden of 7 repeats and low editing efficiencies, but our calculations show that researchers spend about 472 hours of hands-on time and $18,394 in total costs on performing a CRISPR experiment in their lab. See how you compare! We have created a CRISPR Calculator where you can play around with the experimental conditions to calculate the cost of your own CRISPR experiment. FULL STACK GENOME ENGINEERING Products ENGINEERED CELLS KNOCKOUT IPS CELLS KNOCK-IN IPS CELLS KNOCKOUT IMMORTALIZED CELLS KNOCK-IN IMMORTALIZED CELLS ENGINEERED CELL LIBRARIES GET A QUOTE CRISPR KITS GENE KNOCKOUT KIT V2 SYNTHETIC SGRNA GMP SGRNA ADVANCED RNA SCREENING LIBRARIES Learning Center CRISPR METHODS HOW TO GUIDE STEM CELLS PRIMARY CELLS CANCER SICKLE CELL NEWS SOURCES IPSCS GENOME ENGINEERING HISTORY TECHNIQUES & APPLICATIONS Resources RESOURCE LIBRARY THE BENCH BLOG CRISPR CUTS PODCAST HELP CENTER EVENTS Company ABOUT SYNTHEGO PLATFORMS HALO ECLIPSE PARTNERSHIPS CAREERS NEWSROOM CONTACT COPYRIGHT © 2022 SYNTHEGOPRIVACY POLICYTERMS OF USETERMS OF SALEMONEY BACK GUARANTEES Subscribe Feedback